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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is noticed in several tumors, which ends up in chromosome instability, and therefore may well add to tumorigenesis. This protein was found to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a significant position in endorsing mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, happen to be explained. Two supplemental splice variants were being described but specific nucleotide sequence data isn't still out there.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally has the epitope regarded through the antibody.
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This information outlines more achievable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This guideline outlines further more possible optimization of mobile and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By evaluating the staining through the blocked antibody vs . the antibody by itself, you can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic occasion. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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